The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Learn how you can utilize microplate readers to drive landmark discoveries. Well scanning With well scanning, multiple points within each well can be read, providing a high. 312 gain calibration failed. Ensure that cells reach around 80–90% of confluence on the day of the. An example of dual-luciferase assay results. These are the default kind of cuvette & 96 well plate stocked by the lidstrom lab as of 11/2013. Robust shaking and consistent temperature control optimize bacteria and yeast cell growth. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. BMG Labtech - THERMOstar. T = I/I 0 and %T = 100 (T). of 3 wells of a 96-well plate, from 1 of 3 independent experiments. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. p-Nitroaniline - Catalog No. Plate SpectraMaxiD3,iD5,i3x,i3,M2,M2e,M3,M4,M5,M5e,Plus. 000 to 2. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. Features of Clear 96-Well Microplates: • Clear plates for colorimetric ELISA and other assays. During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 000 OD ± 1. #N207) LAL Reagent Water (Lonza cat. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. The soil was amended with either 1. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 5L) MultiWash+. Packed 25 plates per sleeve, 2 sleeves per case. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. 5 ng per well. The. For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro densitometry of TLC plates and electrophoresis gels. 96- and 384-well. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Corning. Users can choose to process all plates within the magazine or select a specific number. EMax. These models can no longer be repaired. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. Published Dec 16,. Compare this item. VIS absorbance detection. 96 well plate injection time with SpectraMax® L plate reader. Three low volume 24-well bottom slides. Some will only support one well density (e. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. Corning. Used. 1. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. When a. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. Another consideration, says Amouretti, is plate format. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. They can be read by most microplate readers. Up to 96 well plates, there is often a good signal that can easily be detected, and 96 wells are measured comparatively quickly. The Best Microplate Readers According to our Product Reviewers (1) Write a Review. Description: Enter plate name (optional)(2) Wild‑type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β‑catenin pathway Molecular medicine reports January 1, 2023 Hu X, Zhang R, Yao J, Mu B, Zhao C. A. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. d. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. $14,995. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Learn more. Temperature variation ± 0. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. 1 – 2. Fluorescence Microplate Reader Comparison Chart. SpectraMax® Microplate Reader Suite of Products Knowledge Base. UV absorbance detection. No accessories are needed and SoftMax® Pro Software. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. S. guaranteed 25 fmol/200 µL fluorescein (125 pM), black 96-well plate; 1 second integration time 15 fmol/75 µL. Dynamic range > 6 decades . This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. Figure 2-23: Bottle holder To install the bottle holder: 1. External Dimensions without lid (mm). The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). Service Contracts, Preventative Maintenance and. 4 (Molecular Devices cat. Microplate Reader. 100 fmol 384-well (75 µL) 0. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Ultraviolet transparent multiwell plates from GreinerThe Accuris SmartReader UV-Vis (model MR9610) is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. 1. The IC50s by cell rounding and LysoTracker. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . The Versamax Microplate Reader is built around a grating monochromator. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. Consequently, FLIPR has been used. SoftMax Pro GxP Software version 5. Eight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. We would like to show you a description here but the site won’t allow us. 1 x plates; Instrument dimensions. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. 5% at 1. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. Site. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. When a. 100 μL of working reagent was added to each well after the 10-minute preincubation step. Figure 3. 0 . 1 fmol luciferase per well. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. Dark blue indicates well will be read. Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. 1. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. #23225). 1. 5 pM 1 0200-7015POS Glow for 96- and 384-well plates 96–well: 1. Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. Polypropylene deep well plates, round bottom. • Remove media from each well using a blue pipette tip being careful as not to remove cells. 5 ng per well. Product Compare 0. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 984. and glow luminescence modes in 96- to 384-well plates. The same volume of genotyping mixture (2x KASP Master mix plus KASP Assay mix) was added to the each well. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. 00. x. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 000 to 2. Access this by going to. explore more. 0 Optical system Filters Power requirementAfter reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. * Note: Programming the washer with a final aspirate height of 4. • Easy and gentle immobilization of biotin. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. M5. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. Select up to 3 products. Optional 12-way manifold) 5032336. With theMolecular DevicesWe designed and fabricated different sized microfluidic covers, matched to the desired experimental conditions: Fig. Materials. 4: Berthold Technologies: TriStar LB 941: Multimodal plate reader: Yes:. The advanced design of the Sunrise plate reader includes. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. This reader also makes use of a unique temperature-independent methodology for path length correction. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical and. The CellTiter-Glo assay was used to measure ATP. 96-well PCR plates are plastic, heat-resistant multi-well microplates designed for use inside PCR machines or real-time (qPCR) thermocyclers. Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. . Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. 340 PC384. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. 4ti-0255 96 well assay plate, pyramid flat bottom, clear, non-sterile UV wavelength 4ti-0214 UltraVision Plate 384, black, non -sterile, with UV baseELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. Cell viability and cytoxicity assays. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. 00. • 0. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. x. The StakMax stacker also allows users to feed plates into the loading area manually. Page 1 of 8 . The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. Absorbance equation. Single or dual wavelength read, kinetic readings, automix, endpoint, and blank pattern, curve fits included with PC Software. The luminescence was measured in a SpectraMax. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. 3 OD at 200 nm. • 0. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. Recertification service. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Bowman Dispensers. White light and fluorescence images of the 6-well plates were captured with the ClonePix 2 System. Absorbance Linearity: 2. Therefore you need to ask a physicist. The CellTiter-Glo assay was used to measure ATP. An industry-renowned instrument for monitoring GPCRs and ion channels. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. Figure 1. • To avoid removing loosely attached cells don’t wash if the background signal is not too highTeams measured absorbance at 600 nm (OD600) and GFP in a plate reader from four technical replicates per biological replicate (for a total of eight replicates and fitting on a 96-well plate) at. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…AquaMax® Microplate Washers are configurable with user interchangeable heads for both 96- and 384-well microplate washing applications, including plate or cell washing. Cell line development teams will realize savings on time and resources, as well as faster time-to-market by utilizing a single technology for measuring a critical quality attribute duringOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 2. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Automated live cell imaging – cell counting and confluence. M2. We would like to show you a description here but the site won’t allow us. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). Reaction formats The MagMAX™-96 DNA Multi-Sample Kit can accommodate a broad range of throughputs. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. 6. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. The plate was preincubated in the reader at 37°C for a minimum of 10 minutes. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. 305 reference level saturation. Cell viability and cytoxicity assays. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. 100 fmol luciferase per well. Anticancer drug assay was also conducted using conventional 96-well plates as controls. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. the Gemini XPS reader. 2. of Dispensers:Molecular Devices deliver high throughput while showing good correlation with manual. x. M5. as well as all other Molecular Devices' microplate readers. Sort By: Show:. 97 independent and fixed optical fibers can achieve a mean time between failures (MTBF) of 20,000 hours, which keeps the microwell plate still during the entire reading cycle,. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. CO 2 & O 2 control. SpectraMax i3x Multi-Mode Microplate Reader. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise:. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. 00. Usage/Application: Lab. Published Dec 16, 2020 | Updated Dec 16, 2020. An additional reference channel for optimizing the light intensity; Auto switch-off of the light source when not in use to extend lifetime; An automated self-check of major components at instrument start-upThe EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. The 8-channel read head allows for fast read times which means a 96-well plate can be read in as little as five seconds. 96-well microplate compatible Well read area range: D4. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. Glass microfiber: Use for cell harvesting and binding assays, liquid scintillation counting or high loading, DNA binding and purification; Melt blown polypropylene: Use for pre-filtration; Cellulose acetate: Use for low protein binding,. Quantitative, whole well cell confluence for every well. Compare specifications and request a quote from leading suppliers. Description. 0 . 0200-6263. Endpoint, kinetic, spectrum. Description Bottom Volume Qty/Pk Qty/Cs 3635 96 Well UV-Plate Flat 370 µL 2550per well of this semi-solid mixture was pipetted into 6-well plates. White plates reflect the light, enabling even low levels of Luminescence to be detected. This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer. 1% in white 96- and < 0. Important things to consider when purchasing a multimode microplate reader. $3,207. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Find our technical support information along with contact details. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. Artel MVS Bundle DM Std/800TSNB Reader Details. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Molecular Devices' microplate readers. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. HeLa. Molecular Devices L-Max II Luminescence Microplate Reader. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. Washers configured for both 96- and 384-well plates. 40266. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. D. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. 993 (Figure 3), validating the assay’s usefulness over a wide range of viable cell numbers. Luminescence applications and assays. Selects either 96-well or 384-well plate for a stand-alone read. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. Dimensions: 18. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. #ABS PLUS) Methods. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. MultiWash+ for 96-well plates: 4 liquid lines, 8-way manifold and tubing, 4 wash/rinse bottles (2L), 1 waste bottle (2. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 25% and 0. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Mode Plate Reader. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Compare. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. 1% in white 96- and < 0. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per. , Corning, NY) or Greiner. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13The microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. QC tools and IQOQ services. Compare Tool. The rate of molecular motion of Protein G slows down when it is bound to antibodies, resulting in an increase of the FP value (Figure 2). 96-, 384-, and 1536-well microplates. Molecular Devices SpectraMax Plus 384 Absorbance. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. x. Place the microplate in the drawer. minutes and begin reading microplates with seven mouse clicks. When the user applies a set temperature of 37°C, the top is heated to 37. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. These lab workhorses are used in drug discovery. The top- and bottom-reading Gemini EM Microplate. 24-, 96-, 384-well plates and microwell strips; Manifold Channels: 96-well washing: 8-well (1x8) manifold, 2x8-well manifold, 12-well (1x12) manifold, 8-well short tube (1x8) manifold. Optional 16-way manifold. Temperature control: 4°C above ambient to 45°C. Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. 96- and 384-well. F3/F5 Multi-Mode Microplate ReadersAnalyst® Multimode Reader—one of the following: • Acquest or Analyst AD or HT with at least v. Suggested plates are black walled. It has a 340-750nm Spectral Range. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 0 OD. This is a Molecular Devices Thermomax 96 well Absorbance Microplate Readers. To help you get the best results from your Molecular Devices® plate readers it is essential that you choose the right plate. High sensitivity and speed are required for these plate formats if you wish to examine quick kinetic measurements such as calcium fluxes or applications with small signal. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Published Dec 16, 2020 | Updated Jun 22, 2023. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. Molecular Devices. In addition, users can define individual reagents and concentrations to be delivered to each well. More Product Information.